Understanding Embryo Grading. Embryologists use a system of grading embryos to determine which are the best for transfer. The most common scoring system was developed in the late 1990s by Dr. David Gardner and is called the Gardner System. This system grades blastocysts starting about day 4-5 in IVF settings. A blastocyst is an embryo that has a fluid filled cavity and is organized into both placenta (trophectoderm) and baby (inner-cell mass).
Blastocyst Development and Quality Grading (or scoring)
There are four distinct parts to a blastocyst – the part that forms the baby (inner cell mass), part that forms the placenta (trophectoderm), the fluid cavity (blastocoele cavity) and the shell (zona pellucida) (see figure above).
As development of blastocysts progresses, cells in the 2 components divide and the fluid cavity enlarges.
With continued blastocyst development the embryo expands, the zona pellucida (ZP) thins, and it hatches from the ZP.
Within the uterus, when the blastocyst hatches it can attach itself to the uterine lining-called implantation.
The Gardner blastocyst grading system has three separate components of quality scores:
Blastocyst development stage – expansion and hatching status (number 1-6)
Inner cell mass (ICM) score, or quality (letter A-D)
Trophectoderm (TE) score, or quality (letter A-D)
Blastocysts are given a quality grade for each of the three components listed above and the score is expressed with the expansion grade (how fluid filled) listed first, the inner cell mass grade listed second and the trophectoderm grade third.
For example, a blastocyst quality grade of 5AB means that the blastocyst is very expanded (grade 5), has many tightly packed cells in the inner cell mass (grade A), and has a trophectoderm with few cells forming a loose epithelium (grade B). Examples of scored blastocytes can be seen below.
Expansiongrade | Blastocyst development and stage status |
1 | Blastocoel cavity less than half the volume of the embryo |
2 | Blastocoel cavity more than half the volume of the embryo |
3 | Full blastocyst, cavity completely filling the embryo |
4 | Expanded blastocyst, cavity larger than the embryo, with thinning of the shell |
5 | Hatching out of the shell |
6 | Hatched out of the shell |
Note: usually grades go to number 6 as embryos are transferred or frozen prior to hatching.
ICM grade | Inner cell mass quality |
A | Many cells, tightly packed |
B | Several cells, loosely grouped |
C | Very few cells |
Note: Some clinics give component cells grades of D or even E in addition to the A, B and C grades described above.
TE grade | Trophectoderm quality |
A | Many cells, forming a cohesive layer |
B | Few cells, forming a loose epithelium |
C | Very few large cells |
Note: Some clinics give component cells grades of D or even E in addition to the A, B and C grades described above.
Blastocyst embryo grading is helpful and often used to select the best embryos to transfer. However, the potential of the embryo to continue normal development, implant and result in a healthy baby born cannot be predicted on grading alone.
There may be some genetic abnormalities. This is why many clinics will recommend the use of pre-implantation genetic testing (often called PGT-A or others). Information on PGT-X testing is provided in other sections.
Examples of early blastocyst grading photos:
 |  |  |
Early blastocyst Early blastocyst 1AB
Very small = Can’t yet grade Very small = Can’t yet grade Cavity embryo’s volume = 1Inner cell mass = Can’t yet grade Inner cell mass = Can’t yet grade Inner cell mass = ATrophectoderm = Can’t yet grade Trophectoderm = Can’t yet grade Trophectoderm = B
Examples of intermediate blastocyst grading pictures:
 |  |  |
2BB 2BC 3BB
Cavity > Cavity > Cavity
1/2 embryo’s volume = 2 1/2 embryo’s volume = 2 completely fills embryo = 3Inner cell mass = B Inner cell mass = B Inner cell mass = BTrophectoderm = B Trophectoderm = C Trophectoderm = B
Examples of expanded blastocyst grading images (typical of day 4-5):
 |  |  |
4AA 4AB 4BB
Well expanded = 4 Well expanded = 4 Well expanded = 4
Inner cell mass = A Inner cell mass = A Inner cell mass = B
Trophectoderm = A Trophectoderm = B Trophectoderm = B
Examples of hatching and hatched blastocyst grading pictures (typically day 5-6):
Picture of a hatching blastocyst on day 6 of in vitro culture
As seen on the lower left side, it is almost hatched from the shell. This hatching will preceed the implantation of the embryo to the uterine lining.
Completely hatched blastocyst
This embryo has a quality grade of 6AA. The tightly packed inner cell mass seen at 3 to 4 o’clock in this picture. A healthy blastocyst will implant within one to four days following a day 5 transfer, with invasion of the cells into the uterine lining occurring soon after blastocyst hatching.
Our blastocyst grading system at the Advanced Fertility Center of Chicago
We use a modified version of the Gardner grading system because our embryologists can not stand to be limited to only 3 grading levels for ICM and TE cells.
The expansion grading is the same as Gardner’s
We use 4 grades, A-D for the ICM and TE
A is the same as Gardner’s A
B is “not quite A, but still quite good”
C is “not quite B, but not bad”
D is for very few cells and/or poor quality cells
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